Researchers at Masaryk University have developed a way to image sexual reproduction in living flowers, according to a study published today in the open-access journal eLife. The new technique, originally reported on bioRxiv, records for the first time movies of fundamental processes in flower development and opens up new avenues for research on plant sexual reproduction.
Plant reproduction occurs in the anthers and ovaries of developing flowers, resulting in the formation of pollen and an embryo sac that holds male and female germ cells. The production of germ cells involves both types of cell division called meiosis and mitosis. Following production, these cells fuse together during fertilisation to produce a cell that develops into a plant+.
Much of our understanding of plant reproductive processes has come from studying dissected samples of plants under a microscope and by examining aberrations arising from mutations in plant genes involved in plant reproduction. However, these methods do not provide information about where and when different events happen during reproduction. Live imaging provides a way of capturing this important detail.
“Live imaging has been instrumental in research into root growth and development, but live-cell imaging of cell processes within the flower is technically much more challenging,” explains co-first author Soňa Valuchová, a postdoctoral researcher at the CEITEC Masaryk University. “There is a need to develop imaging methods in the context of whole organs or plants.”
Valuchova and her colleagues used a technique called light sheet fluorescence microscopy (LSFM), where a sample is moved through a thin sheet of laser light, and a detector picks up three-dimensional image data. In this way, an entire flower that has been embedded in agar can be imaged quickly by moving it through the plane of light. The resulting 3D model shows intricate detail of the flower structure and can be used to track the fate of individual germ cells within it.
Having shown that LSFM could provide high-resolution images of flowers, the team’s next goal was to establish that it could detect specific events in reproduction. To achieve this, they used flowers that had been engineered to have fluorescent labels on key molecules involved in meiosis and mitosis. They were able to capture the entire process of meiosis in male germ cells by detecting changes in the amount and location of a molecule called ASY1 every hour for four days.
The team went on to show that live imaging could be successfully used to study plant hormone levels during different stages of flower development and to watch the movement of chromosomes across the cell during cell division.
One of the most neglected areas of plant reproduction research is the production of female germ cells during female meiosis. Most studies on plant meiosis have focused on male germ cells because the female equivalent – called the megaspore mother cell – are incredibly rare and look very similar to other cells, making them hard to study. To overcome this, the team developed a version of live imaging specifically for female meiosis.
“This required careful dissection of the flower bud to reveal the ovules, which were then passed through the laser light every 10 minutes over 24 hours to create a 3D film,” says co-first author Pavlína Mikulková, also a senior scientist at the Central European Institute of Technology at Masaryk University. “Using this technique, we were able to record the two phases of female meiosis and determine how long each one lasted.”
“This work demonstrates the power of LSFM to provide novel information about plant reproduction that could not previously be studied by other types of microscopy,” concludes group leader and senior researcher Karel Říha, who is also Deputy Director for Research at the CEITEC Masaryk University. “Our success in developing a live imaging protocol for female meiosis represents a major technical advancement in plant cell biology.”